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. 2020 Mar 5;11:1195. doi: 10.1038/s41467-020-14764-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Cross-sectioned retinae of I/R-injured eyes of NOG mice were immunostained with either human CD34 (a, b; hCD34) or human CD31 (c, d; hCD31) antibodies 2 weeks following DVP vs. N-DVP injections. Antibodies for murine collagen type-IV (mCol-IV) were also employed to detect murine blood vessel basement membrane, and murine CD31 (mCD31) detected murine endothelium. The number of b human CD34⁺ or d human CD31⁺ cells detected within transverse layers of the murine neural retina (per 450 μm retinal cross section) was quantitated with imaging software. Each data point represents a replicate individual 450 μm retinal cross section that was analyzed from I/R- treated eyes injected with saline (PBS), primed DVP or N-DVP. Human CD34⁺ or human CD31⁺ endothelial cell engraftment was enumerated in each distinct layer of neural retina shown; only N-DVP migrated into the inner nuclear layer (INL) while most of the primed DVP remained primarily in the superficial ganglion cell layer (GCL). ILM: inner limiting membrane, IPL: inner plexiform layer, outer nuclear layer (ONL), OPL: outer plexiform layer, S: segments. All scale bars = 50 μm. Each individual quantitation shown is an independent experimental measurement with (standard error of mean) SEM from an individually-immunostained cryosection for each of the three groups of injected mouse eyes (i.e., saline-PBS (n = 8), DVP (n = 3, CD31; n = 11, CD34), N-DVP (n = 7, CD31; n = 14, CD34). ***p < 0.001; **p < 0.01; *p < 0.05 (multiple unpaired t-tests).