Fig. 8. Transcriptional profiling of primed vs. naïve VP from normal and diabetic hPSC.

a PCA of whole genome transcriptomes from RNA-Seq samples from primed VP/DVP vs. N-VP/DVP, and their parental isogenic hPSC lines (i.e., primed or naïve hESC-derived VP, primed or naïve hiPSC-derived VP, and primed or naïve DhiPSC-derived DVP). b Heatmap-cluster dendrogram of the top 500 most differentially expressed genes (Supplementary Data 4); hierarchical clustering (Euclidian distance) of isogenic primed vs. naïve VP RNA-Seq samples. Isogenic paired VP samples are same above (n=8; VP/DVP and N-VP/N-DVP). (hESC-derived VP or N-VP: ‘E’; hiPSC-derived VP or N-VP: ‘I’ ; DhiPSC-derived DVP or N-DVP: ‘D’). c Volcano plot of differentially expressed transcripts in whole genome of primed vs. naïve VP; log₁₀ p-values vs. log₂ fold change in expressions. RNA-Seq VP samples are same as in PCA above (n = 8). d GSEA of pathways enriched in primed VP/DVP vs. N-VP/N-DVP. Paired isogenic primed and naive VP samples used for analysis are same as PCA (n = 8).