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. 2020 Mar 5;9(3):31. doi: 10.1038/s41389-020-0216-1

Fig. 4. Suppression of Cdc37 induces immaturity of plasma cells.

Fig. 4

a First panel: The cell culture supernatant of NCI-H929 Scramble and Cdc37shRNA cells was subjected to ELISA analysis for the concentrations of human immunoglobulin light-chain proteins (*p < 0.05). Second panel: Total RNA of NCI-H929 Scramble and Cdc37shRNA cells was isolated and subjected to qRT-PCR analysis for Cdc37 and Xbp1s (**p < 0.01). Third panel: Whole-cell lysates of NCI-H929 Scramble and Cdc37shRNA cells were subjected to western blot analysis for Cdc37 and Xbp1s. Fourth panel: Flow cytometric analysis of surface CD38 and CD49e on NCI-H929 Scramble and Cdc37shRNA cells. b First panel: The cell culture supernatant of KMS11 Scramble and Cdc37shRNA cells was subjected to ELISA analysis for the concentrations of human immunoglobulin light-chain proteins (**p < 0.01). Second panel: Total RNA of KMS11 Scramble and Cdc37shRNA cells was isolated and subjected to qRT-PCR analysis for Cdc37 and Xbp1s (*p < 0.05, **p < 0.01). Third panel: Whole-cell lysates of KMS11 Scramble and Cdc37shRNA cells were subjected to western blot analysis for Cdc37 and Xbp1s. Fourth panel: Flow cytometric analysis of surface CD38 and CD49e on KMS11 Scramble and Cdc37shRNA cells. c NCI-H929 cells were treated with 100 and 200 nM celastrol for 24 and 48 h, respectively. Left panel: The cell culture supernatant was subjected to ELISA for the concentration of human Ig light-chain proteins (*p < 0.05, **p < 0.01, ***p < 0.001). Middle panel: The total RNA was isolated and subjected to qRT-PCR analysis for Cdc37 and Xbp1s (*p < 0.05, **p < 0.01). Right panel: The whole-cell lysates were subjected to western blot analysis for Xbp1s, CD49e, and Cdc37.