Skip to main content
. 2020 Mar 5;9(3):31. doi: 10.1038/s41389-020-0216-1

Fig. 6. Overexpression of Xbp1s partially rescues BTZ resistance caused by Cdc37 suppression.

Fig. 6

a ARP1 cells were infected with empty vector (ARP1 Cdc37 EV) and Cdc37 ORF (ARP1 Cdc37 OE) lentivirus. The gene and protein level of Cdc37 and Xbp1s were detected in ARP1 Cdc37 EV and ARP1 Cdc37 OE cells by qRT-PCR and western blot. b Left panel: The cytomorphological changes of ARP1 Cdc37 EV and ARP1 Cdc37 OE cells. Right panel: The cell culture supernatant was subjected to ELISA analysis for the concentration of human immunoglobulin light-chain proteins (**p < 0.01). c NCI-H929 cells were infected with empty vector (NCI-H929 Xbp1s EV) and Xbp1s ORF (NCI-H929 Xbp1s OE) lentivirus. Left panel: The cytomorphological changes were performed in NCI-H929 Xbp1s EV and Xbp1s OE cells after the treatment with 200 nM celastrol for 72 h. Right panel: NCI-H929 Xbp1s EV and Xbp1s OE cells were treated with 100 and 200 nM celastrol, respectively for 48 h, and the concentration of human Ig light-chain proteins in the culture supernatant was determined by ELISA (*p < 0.05, **p < 0.01, ***p < 0.001). d NCI-H929 Xbp1s EV and Xbp1s OE cells were pretreated with 100 nM celastrol for 48 h, and then were treated with 2 nM BTZ for 24 h. The cells were subjected to apoptosis analysis.