Mechanism of the long-incubation method. (A) Liposome coverage with different incubation times. Analysis was based on 5, 10, and 5 images, 46, 321, and 482 liposomes for E1minG15s, E5minG15s and E10minG15s, respectively. (B) Liposome density when different solutions (Liposome G, liposome-free buffer A, and Liposome E) were used in the second sample application. Analysis was based on 3, 3, and 4 images, 975, 768 and 1,540 liposomes for E10minG15s, E10minBuffer15s, and E10minE15s, respectively. (C) A cartoon showing the long-incubation method.