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. Author manuscript; available in PMC: 2020 Mar 5.
Published in final edited form as: Mol Plant. 2019 Dec 31;13(3):446–458. doi: 10.1016/j.molp.2019.12.013

Figure 5. The Abundance of the Transcripts of CCA1 and LHY Are Regulated by SPY.

Figure 5.

(A) Bioluminescence of CCA1:LUC in transiently transformed N. benthamiana leaves with indicated constructs shown in left panel. 35S:GFP was used as an effecter control.

(B) Quantitative analysis of bioluminescence intensity shown in A. Data represent mean ± SE. (n = 6). ***p < 0.001 (Student t-test).

(C and D) Bioluminescence of CCA1:LUC showing the lower transcription level of CCA1 in spy-3 in continuous red light or continuous darkness as denoted. Data represent mean ± SE (n = 15).

(E and F) Transcript levels of CCA1 (E) and LHY (F) in spy-3 mutant under LD condition. Data represent mean ± s.e. in three biological replicates. The gene expression level was normalized by ACT2 expression.