TABLE 1.

Composition of complete media for HIE maintenance and differentiation

Reagent	Final concentration	Origin
Complete media without growth factors (CMGF−)
Advanced DMEM/F12	N/A	Invitrogen
GlutaMAX-1	2 mM	Invitrogen
HEPES	10 mM	Invitrogen
Penicillin/streptomycin	100 U/ml	Invitrogen
Complete media with growth factors (CMGF+)
CMGF−	N/A	N/A
L-WNT3a-conditioned media	50%	ATCC
R-Spondin-conditioned media	20%	A gift from Dr. Calvin J. Kuo, (Stanford University)
Noggin-conditioned media	10%	A gift from Dr. Gijs van den Brink (University of Amsterdam)
B27	1×	Invitrogen
N2	1×	Invitrogen
N-acetylcysteine	1 mM	Sigma-Aldrich
Mouse recombinant EGF	50 ng/mL	Invitrogen
[Leu15]-Gastrin I	10 nM	Sigma-Aldrich
Nicotinamide	10 mM	Sigma-Aldrich
A-83-01	500 nM	Tocris
SB202190	10 μM	Sigma-Aldrich

Table modified and reproduced with permission from Springer Nature Methods in Molecular Biology DOI 10.1007/7651_2017_1 © Springer Science+Business Media New York 2017.

Winnie Y. Zou, et al Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections [21].