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. 2020 Mar 5;15(3):e0229909. doi: 10.1371/journal.pone.0229909

Fig 3. A. tumefaciens-mediated transformation of ‘Olathe’ pinto bean.

Fig 3

(A) Explants after 4-day co-cultivation in the dark at 25 °C. (I, II) Half-seeds explants. (III) Precultured embryo axes explants. (IV) Embryo axes explants. (B) Precultured embryo axes explants after 3-week selection on selection RM1. (C) Embryo axes explants after 6-week selection on selection RM3. (D, E) GS-resistant calli and green buds induced from the precultured embryo axes explants after 6-week selection on selection RM3. (F) Rooting of the GS-resistant plants produced from the precultured embryo axes explants. Bars = 1 cm.