Table 2. Fluorescence quenching of Sus1 in presence of acrylamide at different pH states.

The ratio of FI in the absence and presence of acrylamide at different concentration was plotted with the acrylamide concentration. Using this plot the Ksv and Kq were calculated.

pH of the protein sample	Ksv [M-1]	Kq [M-1s-1]
10	7.18	1.67 x 10−9
7	6.47	1.50 x 10−9
5	9.02	2.09 x 10−9
3	19.26	4.47 x 10−9
2	21.28	4.94 x 10−9