Fig 4. Evaluation of cadherin-17 expression by confocal fluorescent microscopy and western blotting.

Colonoids were plated on transwell membranes and treated as indicated. a-f: Confocal fluorescence microscopy. After TEER assessment at day-5, membranes were prepared and stained for cadherin-17 expression (max projected). Scale bar = 10 μm. g: Protein isolated from each condition was assessed for cadherin-17 expression by western blotting. 10 μg of protein from each condition was used. β-actin was used as a loading control. Band quantitation was done using ImageJ software. CDH17 = cadherin-17.