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. 2020 Mar 5;15(3):e0229796. doi: 10.1371/journal.pone.0229796

Fig 1. Many [PSI+] variants are cured by DMSO treatment.

Fig 1

A. The indicated strains were plated on 0–10% DMSO gradient plates, and assayed for colony color after growth. Inset shows sectored colonies observed in weak [PSI+] strains. Plate is representative of several trials by independent researchers. B. Weak [PSI+] strains were grown overnight and plated onto rich media plates containing the indicated concentration of DMSO. Each bar shows triplicate trials representing a total of 700 colonies or more. C. Sectored colonies from 4% DMSO were re-streaked on rich media, and assayed for colony color after growth. Images are representative of streaks obtained. D. [PSI+] variants were induced de novo, and a range of color variants were chosen for analysis (variant 1–5; top panel). It should be noted that variant 5 was slightly unstable when plated on rich media. Each variant was cultured in the absence (0%) or presence (5%) of DMSO overnight plated on rich media, and assessed for colony color. The number of colonies counted per treatment is shown.