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. 2020 Feb 18;16(2):e1008337. doi: 10.1371/journal.ppat.1008337

Fig 7. Growth of the M. tuberculosis mmpL4 and mmpL5 mutants under different iron conditions.

Fig 7

Growth curves of Mtb strains including Mtb mc26230 (as WT), ΔmmpL4 (ML2300), ΔmmpL5 (ML2301), ΔmmpL4/L5 (ML2302), ΔmmpS4/S5 (ML859) and ΔmbtD (ML1600) in low-iron 7H9 medium (A) supplemented with 10 μM hemin (B), 1 μM carboxymycobactin (C) and 1 μM mycobactin (D), respectively. The Mtb strains were grown in self-made low-iron 7H9 medium for 5–7 days to deplete intracellular iron before growth assays. The initial OD600 of all the cultures is 0.01. Error bars represent standard deviations from the mean results of biological triplicates.