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. 2020 Feb 24;16(2):e1008083. doi: 10.1371/journal.ppat.1008083

Fig 1. IgG3 Abs exhibit enhanced ADCP despite similar antigen and FcɣR binding affinities.

Fig 1

A. Phagocytic activity of subclass-switched forms of VRC01 (left) and 447-52D (right) antibodies over a range of effector to target (E:T) ratios in the THP-1 ADCP assay using CH505TF gp140 antigen-conjugated beads as target. Error bars indicate SD of duplicates. Dotted horizontal lines represent phagocytosis values observed in the absence of Ab. B-E. Antigen (BaL gp120) (B), FcɣRIIA (C), and FcɣRIIIA (D), FcɣRIIB (E), binding affinity (left, from duplicate assessments of printed antibody spots, bar indicates average/median) and SPR binding profiles (right, raw data in black, kinetic curve fits in red for the ligand concentration series from a representative antibody spot) for VRC01 in IgG1 and IgG3 forms. AU: arbitrary units.