Fig 2. In silico analyses of McmA.

(A) Chromosomal organization of genes near the mcr operon in M. acetivorans. Two SAM-dependent methyltransferases are encoded on either side of the mcr operon. MA4551 encodes a radical SAM methyltransferase that was recently shown to be involved in the conversion of Arg285 in McrA to 5-(S)-methylarginine [27]. We have named this gene mamA based on its proposed role (see text for details). (B) A maximum-likelihood phylogenetic tree of the amino acid sequence of mcmA homologs in archaea. The node labels indicate support values calculated using the Shiomdaira–Hasegawa test using 1,000 resamples. Support values less than 0.6 have not been shown. The outgroup is from bacterial McmA homologs (in black). ANME, anaerobic methane-oxidizing archaea; mamA, methylarginine modification; mcmA, methylcysteine modification; mcr, methyl-coenzyme M reductase; McrA, alpha subunit of MCR; SAM, S-adenosylmethionine.