Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mycobacterium tuberculosis) | H37Rv | (From Ref: 20) | Used at multiple of infection of 0.1 | |
| Strain, strain background (Mycobacterium tuberculosis) | H37Rv pMV306hsp+LuxAB+G13+CDE | (From Ref: 20) | Used at multiple of infection of 0.1 | |
| Strain, strain background (Mycobacterium tuberculosis) | H37Rv pMV306hsp encoding the wild-type FFluc | (From Ref: 20) | Used at multiple of infection of 0.1 | |
| Biological sample (Antibodies) | ||||
| Antibody | anti-CD45-V500 Horizon (Clone no. HI30) | BD Biosciences | Cat.No.563792 | Monoclonal mouse antibody |
| Antibody | anti-CD3 Brilliant Violet 785 (Clone no. OKT3) | Biolegend | Cat.No.317330 | Monoclonal mouse antibody |
| Antibody | anti-CD3-PE (Clone no. HIT3a) | Biolegend | Cat.No.300308 | Monoclonal mouse antibody |
| Antibody | anti-CD4 Brilliant Ultra Violet 496 (Clone no. SK3) | BD Bioscience | Cat.No.612937 | Monoclonal mouse antibody |
| Antibody | anti-CD4-PerCP (Clone no. OKT4) | Biolegend | Cat.No.317432 | Monoclonal mouse antibody |
| Antibody | anti-CD8 Brilliant Violet 605 (Clone no. RPA-T8) | Biolegend | Cat.No.301040 | Monoclonal mouse antibody |
| Antibody | anti-CD8-APC (Clone no. SK1) | Biolegend | Cat.No.344722 | Monoclonal mouse antibody |
| Antibody | anti-CD103-APC (Clone no. Ber-ACT8) | BD Biosciences | Cat.No.563883 | Monoclonal mouse antibody |
| Antibody | anti-CD69 Brilliant Ultra Violet 395 (Clone no. FN50) | Biolegend | Cat.No.310902 | Monoclonal mouse antibody |
| Antibody | anti-HLA-DR-PerCP (Clone no. L243) | Biolegend | Cat.No.307628 | Monoclonal mouse antibody |
| Antibody | anti-CD279-BB515 (Clone no. EH12.1) | BD Biosciences | Cat.No.564494 | Monoclonal mouse antibody |
| Antibody | anti- CD-274-BB515 (Clone no. MIH1) | BD Biosciences | Cat.No.564554 | Monoclonal mouse antibody |
| Antibody | anti-PD-1 Brilliant Violet 421 (Clone no.EH12.1) | BD Biosciences | Cat.No.562516 | Monoclonal mouse antibody |
| Antibody | anti-CD11b-APC (Clone no. ICRF44) | Biolegend | Cat.No.301310 | Monoclonal mouse antibody |
| Antibody | anti-CD45-APC/Cy7 (Clone no. 2D1) | Biolegend | Cat.No.368516 | Monoclonal mouse antibody |
| Antibody | anti-CD14-AP/APC (Clone no. HCD14) | Biolegend | Cat.No.325608 | Monoclonal mouse antibody |
| Antibody | anti-True-Stain Monocyte Blocker | Biolegend | Cat.No.426102 | Monoclonal mouse antibody |
| Antibody | anti-CD4 (Clone no. M7310) | DAKO | Cat.No.M7310 | Monoclonal mouse antibody |
| Antibody | anti-CD8 (Clone no. M7103) | DAKO | Cat.No. M7103 | Monoclonal mouse antibody |
| Antibody | anti-PD1 (Clone no. ab5287) | Abcam | Cat.No.ab52587 | Monoclonal mouse antibody |
| Antibody | anti-TNF-α (Clone no. ab1793) | Abcam | Cat.No.ab1793 | Monoclonal mouse antibody |
| Antibody | Spartalizumab | Selleckchem | Cat.No.A2017 | 20 µg/ml and 200 µg/ml, monoclonal, mouse IgG4 |
| Antibody | IgG4 | Sino Biologicals | Cat.No.13505-HNAH | 20 µg/ml and 200 µg/ml,monoclonal, mouse IgG4 |
| Antibody | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), | Thermo Fisher Scientific | Cat.No.16-4714-82 | 50 µg/ml, mouse monoclonal IgG2A |
| Antibody | Mouse IgG1 Negative Control, clone Ci4 | Merck Life Sciences | Cat.No.MABC002 | 51 µg/ml, mouse monoclonal IgG2A |
| Antibody | anti-TNF-α | Thermo Fisher Scientific | Cat.No.16-7348-81 | 52 µg/ml, mouse monoclonal IgG2A |
| Sequence-based reagent (Applied Biosystems TaqMan Gene Expression primers) |
GAPDH | Thermo Fisher Scientific | #Hs02758991_g1 | |
| β2-Microbulin | Thermo Fisher Scientific | #Hs00608023_m1 | ||
| FNTA | Thermo Fisher Scientific | #Hs00357739_m1 | ||
| PDCD1 | Thermo Fisher Scientific | #Hs01550088_m1 | ||
| CD274 | Thermo Fisher Scientific | #Hs00204257_m1 | ||
| PDCD1LG2 | Thermo Fisher Scientific | #Hs00228839_m1 | ||
| HIF-1α | Thermo Fisher Scientific | #Hs00153153_m1 | ||
| Commercial assay or kit | CytoTox-Glo Cytotoxicity Assay | Promega | G9291 | Commercial assay or kit |
| Lactate Dehydrogenase Activity Assay Kit | Merck | 11 644 793 001 | Commercial assay or kit | |
| Cytokine and Chemokine 35-Plex Human ProcartaPlex Panel | Thermo Fisher Scientific | LHC6005M | Commercial assay or kit | |
| Chemical compound, drug | PD-1/PD-L1 Inhibitor 1 | Cambridge Biosciences, UK | #1675201-83-8 | Chemical compound |
| Recombinant Human G-CSF | ImmunoTools | Cat.No.11343115 | 1 ng/ml and 5 ng/ml | |
| Recombinant Human GM-CSF | ImmunoTools | Cat.No.11343125 | 0.25 ng/ml and 1.25 ng/ml | |
| Recombinant Human IL-1RA/IL1 F3 | ImmunoTools | Cat.No.11344876 | 1.25 ng/ml and 6.25 ng/ml | |
| Recombinant Human IL-10 | ImmunoTools | Cat.No.11340105 | 0.2 ng/ml and 1 ng/ml | |
| Recombinant Human IL-6 | ImmunoTools | Cat.No.11340066 | 10.0 ng/ml and 50.0 ng/ml | |
| Recombinant Human IL-12 | ImmunoTools | Cat.No.11349125 | 0.5 ng/ml and 2.5 ng/ml | |
| Recombinant Human TNF-α | ImmunoTools | Cat.No.11343017 | 0.3 ng/ml, 1.5 ng/ml,7.5 ng/ml and 15 ng/ml | |
| Recombinant Human IL-15 | ImmunoTools | Cat.No.11340155 | 0.5 ng/ml, 5 ng/ml and 50 ng/ml | |
| Recombinant Human IL-17A | ImmunoTools | Cat.No.11340176 | 1 ng/ml, 10 ng/ml and 100 ng/ml | |
| Recombinant Human IL-17F | ImmunoTools | Cat.No.11349176 | 1 ng/ml, 10 ng/ml and 100 ng/ml | |
| Recombinant Human RANTES | ImmunoTools | Cat.No.11343196 | 0.3 ng/ml and 1.5 ng/ml | |
| Recombinant Human MIP-1α | ImmunoTools | Cat.No.11343206 | 1.5 ng/ml and 7.5 ng/ml | |
| Recombinant Human MIP-1β | ImmunoTools | Cat.No.11343223 | 1.0 ng/ml and 5 ng/ml | |
| Recombinant Human MCP | ImmunoTools | Cat.No.11343386 | 1 ng/ml, 10 ng/ml and 100 ng/ml | |
| Software, algorithm | FlowJo | BD Bioscences | version 10.6.1 | Software |
| BD FACSDiva Software | BD Biosciences | Software | ||
| Graphpad Prism | GraphPad Software LLC | v7.05 | Software |