Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | w1118;+;+ | BDSC | RRID:BSDC_3605 | |
| Genetic reagent (D. melanogaster) | Ay-GAL4, UAS-GFP | BDSC | RRID:BDSC_4411 | |
| Genetic reagent (D. melanogaster) | Ay-GAL4, UAS-lacZ(nls) | BDSC | RRID:BDSC_4410 | |
| Genetic reagent (D. melanogaster) | btd-GAL4 | (Estella et al., 2003) | ||
| Genetic reagent (D. melanogaster) | erm-GAL4 | BDSC | RRID:BDSC_40731 | GMR9D11-GAL4 |
| Genetic reagent (D. melanogaster) | GMR71C09-GAL4 | BDSC | RRID:BDSC_39575 | |
| Genetic reagent (D. melanogaster) | insc-GAL4 | (Luo et al., 1994) | GAL4MZ1407 | |
| Genetic reagent (D. melanogaster) | pntP114-94-GAL4 | (Zhu et al., 2011) | ||
| Genetic reagent (D. melanogaster) | wor-GAL4 | (Albertson et al., 2004) | ||
| Genetic reagent (D. melanogaster) | OK371-GAL4 | BDSC | RRID:BDSC_26160 | VGlutOK371 |
| Genetic reagent (D. melanogaster) | tub-GAL80ts | BDSC | RRID:BDSC_7018 | |
| Genetic reagent (D. melanogaster) | UAS-ase | (Brand et al., 1993) | ||
| Genetic reagent (D. melanogaster) | UAS-FLP | BDSC | RRID:BDSC_4539 | |
| Genetic reagent (D. melanogaster) | UAS-FLP | BDSC | RRID:BDSC_4540 | |
| Genetic reagent (D. melanogaster) | UAS-lacZ | (Brand and Perrimon, 1993) | ||
| Genetic reagent (D. melanogaster) | UAS-LT3-NDam | (Southall et al., 2013) | ||
| Genetic reagent (D. melanogaster) | UAS-LT3-NDam-tll | this study | Tll-Dam fusion for Targeted DamID | |
| Genetic reagent (D. melanogaster) | UAS-mCD8-GFP | BDSC | RRID:BDSC_5130 | |
| Genetic reagent (D. melanogaster) | UAS-mCD8-GFP | BDSC | RRID:BDSC_5137 | |
| Genetic reagent (D. melanogaster) |
UAS-mCD8-mCherry | BDSC | RRID:BDSC_27391 | |
| Genetic reagent (D. melanogaster) |
UAS-myr-mRFP | BDSC | RRID:BDSC_7118 | |
| Genetic reagent (D. melanogaster) |
UAS-myr-mRFP | BDSC | RRID:BDSC_7119 | |
| Genetic reagent (D. melanogaster) |
UAS-tll | Kyoto DGRC | 109680 | |
| Genetic reagent (D. melanogaster) | UAS-tll-miRNA[s] | (Lin et al., 2009) | ||
| Genetic reagent (D. melanogaster) |
UAS-tll-shRNA | VDRC | 330031 | |
| Genetic reagent (D. melanogaster) | UAS-TLX | this study | Human TLX under the control of UAS | |
| Genetic reagent (D. melanogaster) | G-TRACE | BDSC | RRID:BDSC_28280 | |
| Genetic reagent (D. melanogaster) | G-TRACE | BDSC | RRID:BDSC_28281 | |
| Genetic reagent (D. melanogaster) | erm-CD4-tdTomato | (Han et al., 2011) | R9D11-CD4-tdTomato | |
| Genetic reagent (D. melanogaster) | erm-mCD8-GFP | (Zhu et al., 2011) | R9D11-mCD8-GFP | |
| Genetic reagent (D. melanogaster) | erm-lacZ | (Haenfler et al., 2012) | R9D11-lacZ | |
| Genetic reagent (D. melanogaster) | Tll-GFP | BDSC | RRID:BDSC_30874 | |
| Genetic reagent (D. melanogaster) | Pnt-GFP | BDSC | RRID:BDSC_42680 | |
| Genetic reagent (D. melanogaster) | FRT82B, tub-GAL80 | BDSC | RRID:BDSC_5135 | |
| Genetic reagent (D. melanogaster) | FRT82B | BDSC | RRID:BDSC_2035 | |
| Genetic reagent (D. melanogaster) | FRT82B,tlll49/TM6B | (Pignoni et al., 1990) | ||
| Genetic reagent (D. melanogaster) | dpn>KDRTs-stop-KDRTs>GAL4 | (Yang et al., 2016) | ||
| Genetic reagent (D. melanogaster) | ase-GAL80 | (Neumüller et al., 2011) | ||
| Genetic reagent (D. melanogaster) | stg14-kd | (Yang et al., 2016) | ||
| Antibody | rabbit anti-Ase (polyclonal) |
(Brand et al., 1993) Gift from the Jan Lab |
IF 1:2,000 | |
| Antibody | chicken anti-β-Galactosidase (polyclonal) |
abcam | ab9361 | IF 1:1,000 |
| Antibody | rabbit anti-β-Galactosidase (polyclonal) |
Cappel (now MP Biomedicals) | 55976 | IF 1:10,000 |
| Antibody | guinea pig anti-Dpn (polyclonal) |
(Caygill and Brand, 2017) | IF 1:5,000 | |
| Antibody | rat anti-Elav (monoclonal) | DSHB | 7E8A10 conc. | IF 1:100 |
| Antibody | chicken anti-GFP (polyclonal) |
abcam | ab13970 | IF 1:2,000 |
| Antibody | rabbit anti-PntP1 (polyclonal) |
A gift from Jim Skeath | IF 1:500 | |
| Antibody | mouse anti-Pros (monoclonal) |
DSHB | MR1A | IF 1:30 |
| Antibody | rabbit anti-Tll (polyclonal) |
(Kosman et al., 1998) Asian Distribution Center for Segmentation Antibodies |
IF 1:300 |