Fig. 3.

High glucose promotes cells damage, apoptosis and oxidative stress induced by hypoxia/reoxygenation injury in vitro.

A. Western blot analysis showing protein expression of KIM-1; B. Western blot analysis showing protein expression of cleaved caspase3; C. Flow cytometry assay. D. Quantitative real-time PCR detected the mRNA levels of TNF-α, IL-1β, IL8 and MCP-1; E. Protein and F. mRNA levels of NOX4; G. ROS was assessed by the detection of 2′,7′-dichlorodihydrofluorescein (DCF) and dihydroethidium (DHE) fluorescence. Scale bars = 100 μm. Data represent the mean ± S.E.M. for at least 3–4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 compared to Normoxic group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 compared to NG group. NG: 5.5 mM glucose plus mannitol 24.5 mM mannitol; HG: high glucose; H/R: hypoxia/reoxygenation; HH/R: H/R under HG condition.