Fig. 6.

Disruption of TGF-βRII ameliorates HH/R-induced cell damage, apoptosis, inflammation and oxidative stress.

A and B. Western blot analysis showing protein expression of KIM-1(A) and cleaved caspase-3 (B); C. Flow cytometry assay; D. Quantitative real-time PCR detected the mRNA levels of MCP-1 and IL-1β; E. Western blot analysis showing protein expression of NOX4; F. DCF and DHE staining of ROS level. Scale bars = 100 μm. Data represent the mean ± S.E.M. for at least 3–4 independent experiments. *P < 0.05, ***P < 0.001 compared to NG group; ^###P < 0.001 compared to HG group; ^ϕϕϕP < 0.001 compared to H/R group. ^$$$P < 0.001 compared to Wild Type group. NG: 5.5 mM glucose plus mannitol 24.5 mM mannitol; HG: high glucose; H/R: hypoxia/reoxygenation; HH/R: H/R under HG condition.