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. 2020 Mar 5;4(1):016105. doi: 10.1063/1.5122804

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© Author(s).

2473-2877/2020/4(1)/016105/11

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FIG. 2. — Cord patches become perfused at 3 days post-implantation. (a) H&E and (b) Sirius Red/Fast Green staining of cord patches explanted at 1, 3, and 7 days indicates pooling of blood around cords at day 3, followed by remodeling into micro-vessels (arrowheads, day 7). Scale bar = 20 μm, and inset scale bar = 10 μm. (c) CD68+ cells are prevalent at all timepoints, remaining elevated in the patch at 7 days. The yellow line indicates the patch boundary. Scale bar = 100 μm. (d) Hematoxylin positive nuclei within the patch per 100 μm². (^***p < 0.001). (e) Quantification of the collagen area from Sirius Red stain. (^***p < 0.001). (f) Quantification of the CD68+ area (^*p < 0.05). Error bars indicate S.E.M., n = 3 (day 3, day 7), and n = 1 (day 1).