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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Cancer Discov. 2019 Dec 6;10(3):460–475. doi: 10.1158/2159-8290.CD-19-0837

Figure 4. Loss of TBK1 selectively suppresses VHL null ccRCC tumor growth.

Figure 4.

A-C, Immunoblots of lysates (A) from, representative crystal violet staining (B) and 3-D soft agar growth pictures (C) of UMRC2 cells infected with lentivirus encoding either inducible Ctrl shRNA or inducible TBK1 shRNA and then treated with doxycycline as indicated.

D-E, Representative bioluminescence imaging of before (0 week) and 10 weeks post-doxycycline treatment (D) and quantification of post-doxycycline treatment bioluminescence imaging (E) from UMRC2 luciferase stable cells infected with lentivirus encoding either Teton-control (Ctrl) Teton-shTBK1 3185 (shTBK1) injected orthotopically into the renal capsule of NSG mice. Two-way ANOVA analysis was performed for E.

F, Quantification of kidney tumor weight (n=12).

G-H, Representative lung ex vivo bioluminescence imaging (G) and quantification of ex vivo imaging (H, n=12).

Error bars represent SEM, *P<0.05, **P<0.01, ***P<0.001