(A) Schematic showing different delivery methods of β-catenin DsiRNA in Mdr2 KO mice. (B) qPCR shows significant decrease in Mdr2 gene expression in KO liver with and without β-catenin inhibition as compared to WT. (C) IF demonstrates almost complete reduction of β-catenin in hepatocytes in WT liver following its knockdown (200X). (D) Liver weight/body weight (LW/BW) ratio remains unchanged in KO/KD compared to Mdr2 KO. However, KO/KD had significantly higher serum ALP, ALT, AST, total bilirubin, and conjugated bilirubin levels compared to Mdr2 KO alone. **p<0.01; ****p<0.0001.