Fig. 7.

Effects of in vivo overexpressed SORBS2 on cardiac structure and function in mice. (a) and (b) Immunofluorescence staining against β-tubulin (scale bars: 10 μm). Statistical analysis of the β-tubulin quantitative analysis levels in mice tissues. (n = 5 for each group, ** p < 0.01; Student's t-test). Data shown as the mean ± SEM. (c) Representative images of JP2 immunofluorescence staining of mice tissues. Red arrows mean that JP2 moved to merge in cardiac tissues from harvested mice injected with SORBS2 overexpression AAV9 vectors. (d) Immunofluorescence staining against T-tubule with WGA in mice tissues. (scale bars: 10 μm). (e) Representative cytosolic Ca²⁺ images (upper) and traces (lower) in freshly isolated cardiomyocytes from hearts transfected with empty virus and SORBS2 overexpression virus. (f) and (g) Average data of the amplitude and time to peak of Ca²⁺ transients. (n = 6–10 cells from one heart, n = 5 each group). (h) The statistical analysis of evaluation of time to calcium decay 50%.