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. 2020 Feb 28;11:50. doi: 10.3389/fpls.2020.00050

Figure 2.

Figure 2

Arabidopsis VDACs differentially complement Δpor1 yeast in complementation assay. (A) Δpor1 transformed with different VDACs were tested in a drop-serial dilution assay. Cells were plated on media containing 2% glucose (YPD) or 3% glycerol (YPG) as the sole carbon source and incubated at 30°C and 37°C for 5 days and then their growth was documented. VDAC1, VDAC2 and VDAC4 enable Δpor1 to grow on glucose and glycerol at 37°C. A partial recovery is also observed in the case of VDAC3. (B) Growth kinetics of the Δpor1 transformed with different VDACs in YPD and YPG at different temperatures. The data shown are the mean of three independent biological replicates ± SD. (C) Western blot analysis of mitochondrial lysates of Δpor1 yeast strain expressing (empty) pGV8 and Arabidopsis VDAC1, VDAC2, VDAC3, and VDAC4. The antibody anti-AtVDAC1 (1:5,000, Agrisera) was used to identify VDAC1, VDAC2, VDAC3, and VDAC4 and anti-Mtg2p (1:2,000) yeast antibody was used as a mitochondrial marker. Arabidopsis VDAC1, VDAC2, VDAC3, and VDAC4 proteins were expressed and directed to mitochondria of Δpor1 yeast cells.