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. 2020 Feb 28;11:50. doi: 10.3389/fpls.2020.00050

Figure 4.

Figure 4

Effect on MMP due to complementation and overexpression of VDACs. (A) Confocal microscopy of M3 and Δpor1 cells. M3 shows higher uptake of DASPMI than Δpor1. White scale bar in 5 μm. (B) Confocal microscopy of Δpor1 cells complemented with VDACs. White scale bar in 5 μm. (C) Confocal microscopy of M3 cells overexpressing VDACs. White scale bar in 5 μm. (D) MMP measurement in Δpor1 cells transformed with VDAC (1, 2, 3, and 4) grown in 3% YPG by flow cytometry. The Δpor1 shows lower DASPMI positive cells than M3 when grown in YPG. Complementation of VDACs (1, 2, 3, and 4) in Δpor1 results in higher DASPMI positive cells restoring the mitochondrial health. The data shown are the mean of three independent biological replicates ± SD. Student's t-test (**) P < 0.001 related to the Δpor1 pGV8. (E) MMP measurement in M3 cells transformed with VDAC (1, 2, 3, and 4) grown in 3% YPG by flow cytometry. Overexpression of VDACs (1, 2, 3, and 4) in M3 further increases the DASPMI positive cells. The data shown are the mean of three independent biological replicates ± SD. Student's t-test (*) P < 0.001 related to the M3pGV8.