FIGURE 9.
ORF5 protein promotes PCV2 replication via mTOR-ERK1/2-AMPK. (A) PK-15 cells were pretreated with U0126, AICAR, Compound C and Rapa for 2 h and then infected with PCV2 or PCV2ΔORF5. At 36 h post-infection, PCV2 DNA copies were quantified by quantitative PCR. (B) PK-15 cells were pretreated with U0126, AICAR, Compound C and Rapa for 2 h and then infected with PCV2 or PCV2ΔORF5. At 36 h post-infection, Cap protein and GFP protein were detected by immunoblotting. (C) Perform a T-test on the grayscale analysis of the (B), the ratio of Cap to β-actin. *p < 0.05, **p < 0.01.