FIGURE 5.

ORF5 protein upregulates phosphorylation of AMPK. (A) PK-15 cells were transfected with EGFP-ORF5 or EGFP-C1 for 48 h. AICAR was used as a positive control. p-AMPK and t-AMPK were detected by immunoblotting. (B) Perform a T-test on the grayscale analysis of the (A), the ratio of targeted protein to β-actin. (C) PK-15 cells were pretreated with Compound C for 2 h and then transfected with EGFP-C1, EGFP-ORF5. After 48 h, immunoblot was used to detect p-mTOR, t-mTOR, p-AMPK, t-AMPK, p-ERK1/2, t-ERK1/2, LC3II were detected by immunoblotting 48 h post-transfection. (D) T-test on the grayscale analysis of the (C), the ratio of targeted protein to β-actin. *p < 0.05, **p < 0.01.