FIGURE 6.

Laser confocal microscope detection of point-like aggregation of LC3. LC3 was detected by laser confocal microscope. (A) PK-15 cells were co-transfected with DsRed-ORF5 and EGFP-LC3 for 48 h. (B) PK-15 cells were transfected with DsRed-ORF5. (C) PK-15 cells co-transfected with DsRed-N1 and EGFP-LC3. (D) PK-15 cells were transfected with DsRed-N1. (E) Control group. (F) PK-15 cells were pretreated with GSK2606414, then co-transfected with DsRed-ORF5 and EGFP-LC3. (I) PK-15 cells were pretreated with Rapa, then co-transfected with DsRed-ORF5 and EGFP-LC3. (G) PK-15 cells were pretreated with Compound C, then co-transfected with DsRed-ORF5 and EGFP-LC3. (K) PK-15 cells were pretreated with AICAR, then co-transfected with DsRed-ORF5 and EGFP-LC3. (L) PK-15 cells were pretreated with U0126, then co-transfected with DsRed-ORF5 and EGFP-LC3. ^∗p < 0.05, ^∗∗p < 0.01.