FIGURE 7.

ORF5 protein promotes replication of PCV2. (A) PK-15 cells were transfected with EGFP-ORF5 for 12 h and then infected with PCV2 for 36 h. PCV2 DNA copies were quantified by quantitative PCR. (B) PK-15 cells were pretreated with TG or 4-PBA for 2 h and then transfected with EGFP-ORF5 or infected with PCV2 for 36 h. DNA copies were quantified by quantitative PCR. (C) PK-15 cells were pretreated with 4-PBA for 2 h and then transfected with EGFP-ORF5 for 12 h and then infected with PCV2. At 36 h post-infection, Cap protein and GFP protein were detected by immunoblotting. (D) T-test on the grayscale analysis of the (C), the ratio of Cap to β-actin. *p < 0.05, **p < 0.01.