Table 1.
Differentially expressed genes being targets of miR-221/222 and belonging to the GO cluster “cation channel complex” and “T-tubule”
| Gene name | RefSeq | Protein | Cation channel complex | T-tubule | WT mean | WT SD | KO mean | KO SD | LogFC_WT-KO | FDR_WT-KO |
|---|---|---|---|---|---|---|---|---|---|---|
| Abcc9 | NM_021042 | ATP-binding cassette, sub-family C (CFTR/MRP), member 9 | x | 324.8 | 22.0 | 205.2 | 24.8 | − 0.66 | 9.06E − 07 | |
| Adra1a | NM_001271760 | Adrenergic receptor, α1a | x | 25.5 | 2.2 | 12.2 | 2.3 | − 1.03 | 3.61E − 09 | |
| Cacna1c | NM_001256002 | Calcium channel, voltage-dependent, L-type, αC subunit | x | x | 297.3 | 24.4 | 215.0 | 20.8 | − 0.46 | 1.39E − 03 |
| Cacna1 h | NM_001163691 | Calcium channel, voltage-dependent, T-type, α1H subunit | x | 7.2 | 1.6 | 2.6 | 0.8 | − 1.50 | 5.68E − 07 | |
| Cacna1 s | NM_014193 | Calcium channel, voltage-dependent, L-type, α1S subunit | x | x | 6.9 | 1.0 | 3.9 | 1.0 | − 0.78 | 8.74E − 03 |
| Cacna2d1 | NM_001110844 | Calcium channel, voltage-dependent, α2/δ subunit 1 | x | x | 55.9 | 3.3 | 40.6 | 5.6 | − 0.46 | 1.10E − 02 |
| Cacnb2 | NM_001309519 | Calcium channel, voltage-dependent, β2 subunit | x | x | 64.9 | 9.0 | 40.3 | 5.2 | − 0.69 | 7.26E − 05 |
| Cntn2 | NM_177129 | Contactin-2 | x | 3.5 | 1.1 | 1.1 | 0.3 | − 1.49 | 1.13E − 03 | |
| Herpud1 | NM_022331 | Homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1 | x | 49.3 | 10.7 | 33.3 | 3.2 | − 0.56 | 2.20E − 02 | |
| Kcna1 | NM_010595 | Potassium voltage-gated channel, shaker-related subfamily, member 1 | x | 2.2 | 0.8 | 0.7 | 0.3 | − 1.63 | 1.20E − 02 | |
| Kcnc3 | NM_001290682 | Potassium voltage-gated channel, Shaw-related subfamily, member 3 | x | 2.8 | 0.7 | 1.2 | 0.6 | − 1.02 | 2.66E − 02 | |
| Kcnd2 | NM_019697 | Potassium voltage-gated channel, Shal-related family, member 2 | x | x | 15.0 | 1.9 | 7.5 | 1.1 | − 1.02 | 5.25E − 07 |
| Kcnip2 | NM_001276358 | Kv channel-interacting protein 2 | x | 53.7 | 2.9 | 34.8 | 5.2 | − 0.62 | 9.74E − 05 | |
| Kcnj2 | NM_008425 | Potassium inwardly-rectifying channel, subfamily J, member 2 | x | x | 25.0 | 5.8 | 15.8 | 1.9 | − 0.64 | 1.86E − 02 |
| Kcnj3 | NM_008426 | Potassium inwardly-rectifying channel, subfamily J, member 3 | x | x | 15.4 | 2.0 | 7.0 | 3.8 | − 1.23 | 1.28E − 03 |
| Kcnj5 | NM_010605 | Potassium inwardly-rectifying channel, subfamily J, member 5 | x | x | 23.8 | 4.4 | 12.3 | 3.0 | − 0.96 | 1.30E − 04 |
| Scn4a | NM_133199 | Sodium channel, voltage-gated, type IV, α | x | 24.3 | 2.6 | 12.9 | 2.6 | − 0.95 | 1.24E − 06 | |
| Shisa6 | NM_001034874 | Shisa family member 6 | x | 2.1 | 1.4 | 0.3 | 0.3 | − 2.35 | 1.61E − 03 | |
| Ryr2 | NM_023868 | Ryanodine receptor 2, cardiac | x | 1685.2 | 156.6 | 1110.4 | 44.7 | − 0.60 | 5.21E − 08 |
Among those, genes were the three subunits for the cardiac L-type Ca2+ channel (Cacnb2, Cacna1c, Cacna2d1), a voltage-gated potassium channel (Kcnd2), and both subunits of the acetylcholine-activated, inwardly-rectifying potassium channel (GIRK4, Kcnj5 and GIRK1, Kcnj3)
N = 6 animals/group, WT wild type, KO knockout, SD standard deviation, logFC logarithmic fold-change, FDR false discovery rate, amounts for WT and KO are given as fragments per million