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. 2020 Mar 6;11:1162. doi: 10.1038/s41467-020-14929-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic and functionality. The side inset shows the effect of VIFFI on the formed images of cells on the sCMOS camera. The upper inset shows scanned light-sheet excitation of cells flowing in the microfluidic channel. The motion of a flowing cell is virtually “frozen” on the camera by precisely canceling the motion in order to increase the exposure time of the image sensor and form a fluorescence image of the cell with 1000-fold increase in the camera’s integration time. b Comparison in image acquisition on the sCMOS camera with and without the beam scan. c Serial image frames acquired by the sCMOS camera.