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. 2020 Mar 5;11(3):172. doi: 10.1038/s41419-020-2359-6

Fig. 2. Deletion of Id3 increases prostaglandin E2 (PGE2) secretion in BMMSCs through BMP4.

Fig. 2

a Principal components analysis (PCA) and volcano graphic showing differences in gene expression between bone marrow-derived mesenchymal stem cells (BMMSCs) form WT mice and Id3−/− mice (WT and Id3 KO), analyzed by Affymetrix gene chip arrays (n = 3 per group). b Differentially expressed genes were analyzed by qPCR. c Effects of Bmp4, Cxcl12, Nov, Ms4a4a, Prg4 knockdown by siRNA on prostaglandin E2 (PGE2) secretion levels in in supernatant from WT BMMSCs and Id3−/− BMMSCs; negative siRNA was transfected as control group (Ctrl). d Effects of Lce1h and Serpinb2 knockdown by shRNA on PGE2 levels in WT BMMSCs. e Western blot of ID3, BMP4, and COX2 expression in WT and Id3−/− BMMSCs. f–i Effect of Id3 siRNA transfection of WT BMMSCs on Id3, Bmp4, Cox2, and Ptgs2 mRNA levels, analyzed by qPCR. j Western blot of BMP4 and COX2 levels in Id3 siRNA and control group. k Western blot of BMP4 level after transfection of WT BMMSCs with Id3 overexpressing viral vector; blank vector was transfected as control group. m Western blot of BMP4 expression in human BMMSCs transfected with ID3 siRNA. Values are means ± SD (n = 5, three independent experiments). Student’s t tests and one-way ANOVA. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.