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. 2020 Mar 5;11(3):172. doi: 10.1038/s41419-020-2359-6

Fig. 3. ID3 regulates BMP4 by preventing E2A binding to Bmp4 promoter.

Fig. 3

a Co-immunoprecipitation analyses of binding of ID3 to E2A in bone marrow-derived mesenchymal stem cells (BMMSCs). b Western blot analyses the BMP4 expression after E2A overexpression in BMMSCs. c Western blot analyses the BMP4 expression after E2A knockdown by E2A siRNA in BMMSCs. d Western blot analyses the BMP4 expression in BMMSCs from E2A−/− mice. e Prediction of E2A binding site in the Bmp4 promoter. f–h Chromatin immunoprecipitation analyses of E2A binding after Id3 knockdown (BMMSCs from WT mice and Id3−/− mice), Id3 overexpression (HA labeled vector and Id3 transfected WT BMMSCs), and E2A overexpression (Flag labeled vector and E2A transfected WT BMMSCs). Values are means ± SD (n = 3, three independent experiments). Student’s t tests and one-way ANOVA. *P ≤ 0.05; **P ≤ 0.01.