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. 2020 Feb 28;11:97. doi: 10.3389/fphar.2020.00097

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Copyright © 2020 Zheng, Liu, Zheng, Zheng, Zhang, Zheng, Sun, Chen, Yang, Wang, Lin, Chen, Zhou, Zheng, Xu and Ying

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Maternal 25-hydroxyvitamin D deficiency decreases the antioxidation ability in offspring liver and pancreas tissues. (A) The liver and pancreas ROS levels in offspring at week 0 or 16 was assessed by flow cytometry using the CM-H2DCFDA fluorescence probe. (B, C) The percentage of fluorescence intensity was calculated as relative ROS level in liver (B) and pancreas (C) at week 0 and 16. (D) SOD levels were detected in the offspring liver and pancreas at week 0 and 16. Mean ± SD, n = 5 rats per group. *P < 0.05, **P < 0.01, vs. control group. Normal group, Con; maternal 25-hydroxyvitamin D deficiency group, VDD; week, w.