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. 2020 Feb 28;11:97. doi: 10.3389/fphar.2020.00097

Figure 5.

Figure 5

Maternal 25-hydroxyvitamin D deficiency altered expression levels of Nrf2, CBR1, and inflammatory cytokine in offspring at week 0 and 16. (A) Nrf2 and CBR1 immunohistochemical staining in offspring liver and pancreas tissues obtained in normal group (Con) and 25-hydroxyvitamin D deficiency group (VDD) at week 0 and 16 (magnification, ×200). (B, C) Western blotting analysis was used to detect theNrf2 and CBR1 expression level at week 0 and 16 in offspring rat liver (B) and pancreas (C). Densitometry data for Nrf2 and CBR1 from the blots shown were normalized for analysis to GAPDH, the loading control. (D, E) The Nrf2 and CBR1 levels in offspring liver (D) and pancreas (E) at week 0 and 16 were determined by qRT-PCR. (F) IL-1β, IL-6and TNF-α IH staining in offspring liver and pancreas tissues obtained in normal group (Con) and 25-hydroxyvitamin D deficiency group (VDD) at week 0 and 16 (magnification, ×200). Mean ± SD, n = 5 rats per group. ***P < 0.01, vs. control group. Normal group, Con, Con-1, and Con-2 represent different rats; maternal 25-hydroxyvitamin D deficiency group, VDD, VDD-1, and VDD-2 represent different rats; week, w.