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. 2020 Feb 28;11:363. doi: 10.3389/fimmu.2020.00363

Figure 1.

Figure 1

DC restricted precursor maintain a highly proliferative state in obesity. (A) Phenotypic definition and gating strategy of BM progenitors. (B) Enumeration of progenitors in diet-induced obese mice (HFD) compared with chow fed (CF) control mice. n = 10/group. (C) Mean percentages of EdU (5-ethynyl-2′-deoxyuridine) showing proliferation of BM CDPs from EdU-treated CF and HFD mice. n = 5/group. Two independent experiments. Results are expressed as mean ± s.e.m. (D) Representative FACS plot and (E) mean percentages of cell-cycle profile showing CDPs from CF and HFD mice. n = 5 mice/group. (F) qRT-PCR analyses for G1-cyclins (D2, E1), Ckis (p21), and S-G2/M-cyclins (B2, G1, and F) gene expression in BM CDPs isolated from CF and HFD mice. Two independent experiments. Results are expressed as fold change ± s.e.m normalized to β-actin expression. n = 4/group. Student's t-test *P < 0.05.