Figure 3.

Obesity increases the severity of airway inflammation. (A) Timeline for sensitization and challenge. CF or HFD mice were immunized with HDM, as indicated. i.n., intranasal; i.p., intraperitoneal. (B) Differential cell counts and (C) CCL24 in BAL. (D) Plasma levels of HDM-specific IgE and IgG1 assessed by ELISA. (E) Enumeration of total T cells and Tregs from lung draining mediastinal lymph nodes. (F) Hematoxylin and eosin (H & E) and periodic acid-schiff (PAS)-stained lung sections (Scale bars 100 μm for 100x images). (G) Lung cytokine levels were measured by means of ELISA. Data are representative of two independent sets of experiment and expressed as mean ± s.e.m. n = 8–10/group. One-way ANOVA with Sidak's multiple comparison test *P < 0.05. Eos, Eosinophils; AM, Alveolar macrophage; PMN, Neutrophils; Lym, Lymphocytes; Tregs, T regulatory cells.