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. 2020 Feb 28;11:363. doi: 10.3389/fimmu.2020.00363

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Copyright © 2020 Jaiswal, Makhija, Stahr, Sandey, Suryawanshi, Saxena, Dagur, McCoy, Levine and Mishra.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Obesity alters myeloid cell accumulation in airway inflammation. Naïve HFD and CF mice were sensitized, as mentioned in Figure 3. One day after the last HDM sensitization, lungs were analyzed for different subsets of antigen presenting myeloid cells. (A) FACS plot show the gating strategy. Blue arrows show the gating hierarchy. (B) Bar graph showing mean percentages of CDPs in the BM and (C) number of alveolar macrophages, (D) interstitial macrophages, conventional (E) CD103⁺ DCs, and (F) CD11b⁺ DCs and (G) plasmacytoid DCs in the lung. Data are representative of two independent sets of experiment and expressed as mean ± s.e.m. n = 8–10/group. One-way ANOVA with Sidak's multiple comparison test. *P < 0.05. AMΦ, Alveolar Macrophage; IMΦ, Interstitial Macrophage; pDCs, Plasmacytoid DCs.