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. 2020 Feb 28;11:296. doi: 10.3389/fimmu.2020.00296

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Copyright © 2020 Whalley, Dolton, Brown, Wall, Wooldridge, van den Berg, Fuller, Hopkins, Crowther, Attaf, Knight, Cole, Peakman, Sewell and Szomolay.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recognition of 72 randomly chosen and uniformly distributed fungal peptides by the InsB4 T-cell clone. Each panel shows results of MIP-1β assay, indicating activation of T-cell clone InsB4 by 12 peptides and the index HLVEALYLV peptide (shown as a bold red line). InsB4 was incubated overnight with the indicated peptides and MIP-1β released into the supernatants quantified by ELISA. Bins 1–5 were performed in the same assay with Bin 6 performed in a separate experiment hence the different EC₅₀ values for the index peptide. EC₅₀ for each peptide (M) is displayed in the tables. NR, no T-cell response. The mean pEC₅₀ for each bin is plotted in Figure 7A.