Figure 9.

InsB4 recognizes bacterial peptides with increased sensitivity relative to index insulin peptide. The 20 top-scoring peptides from category II (i.e., confirmed pathogenic) bacterial species were tested overnight for activation on InsB4 by MIP-1β ELISA. The likelihood scores and EC₅₀ of peptide (M) activation are shown. Putative proteins are the product of predicted open reading frames from the genome sequence of the species indicated. Bold EC₅₀ more potent than the index peptide. ~ = ambiguous EC₅₀ based on GraphPad Prism software analysis. Amino acid residues that are the same for each bacterial peptide and the insulin index peptide (HLVEALYLV) are underlined.