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. 2020 Feb 4;20:62–72. doi: 10.1016/j.omtn.2020.01.032

Figure 1.

Figure 1

KCNQ1OT1 Is Downregulated in the VSMCs of Mice with Intimal Hyperplasia and in the Process of VSMC Proliferation

The vein graft model (VG) was constructed in mice (n = 25) to introduce intimal hyperplasia. (A and B) After 0, 1, 2, 3, and 4 weeks (n = 5 at each time point), (A) the intimal area was calculated by subtracting the luminal area from the area within the internal elastic lamina, and (B) the expression of KCNQ1OT1 in isolated VSMCs was detected using quantitative real-time PCR. VSMCs were isolated from the normal mice and stimulated with PDGF-BB with an increased concentration gradient (0, 5, 10, and 20 ng/mL) for 48 h. (C) The expression of KCNQ1OT1 in VSMCs was determined using quantitative real-time PCR. VSMCs were treated with PDGF-BB (10 ng/mL) for different durations (24, 48, and 72 h). (D) The expression of KCNQ1OT1 was examined by quantitative real-time PCR. *p < 0.05 and **p < 0.01 compared with the 0 time point or without PDGF-BB.