Figure 2.

Overexpression of KCNQ1OT1 Suppresses VSMC Proliferation, Migration, and Secretion of Inflammatory Factors

In VSMCs treated with PDGF-BB (10 ng/mL, 48 h), KCNQ1OT1 was overexpressed by transfecting the Ad-KCNQ1OT1 vector, with Ad-GFP acting as the negative control. (A) The proliferation of VSMCs was evaluated using the BrdU staining method. (B) VSMC migration was assessed by transwell assay. (C) The level of inflammatory factors, such as IL-1β, IL-6, and TNF-α, in VSMC culture supernatant was determined using ELISA. *p < 0.05 and **p < 0.01.