Fig. 1.
Bortezomib markedly induces HSP70 expression in multiple myeloma. a qRT-PCR analysis of the expression HSP70, HSP90, GRP-78, and HSP27 in bortezomib-treated RPMI 8226, MM.1S, and OPM2 cell lines. Cells were cultured with vehicle or 5-nmol/L bortezomib for 24 h and then harvested for RNA extraction, cDNA synthesis, and PCR analysis. The vertical axis indicates mRNA expression levels (*p < 0.05, **p < 0.01, ***p < 0.001). b Bortezomib activates HSPS, markedly induces HSP70 expression in MM cell lines. Cells were cultured with vehicle or 2.5-, 5-, and 10-nmol/L bortezomib for 24 h and then harvested and probed with the indicated antibodies by western blotting analysis. c Grayscale of the detected proteins on the above were analyzed by ImageJ software. These experiments were repeated in triplicate