Fig. 6.

Changes of CHOP by bortezomib and VER-155008 combination treatment. a. qRT-PCR analysis of CHOP expression in MM.1S cells. Cells were cultured with vehicle, bortezomib (2.5 nmol/L), VER-155008 (2.5 μmol/L), and the combination therapy for 48 h and then harvested for RNA extraction, cDNA synthesis, and PCR analysis. The vertical axis indicates mRNA expression levels (***p < 0.001). b After cells were cultured with different treated groups mentioned above, CHOP expression was detected by western blotting. c Grayscale of the CHOP protein was analyzed by ImageJ software. These experiments were repeated in triplicate