Fig. 4.

C. sakazakii induced endoplasmic reticulum (ER) stress in mouse models and in BMECs. The mammary gland tissues of each group (n ≥ 3) were obtained at 24 h after C. sakazakii and PBS infection. a, c Grp78 protein and Chop protein were detected by western blot in mice and in BMECs. b, d The relative Grp78 protein and Chop protein levels were quantified by ImageJ and normalized to α-Tublin and GAPDH in mice and BMECs. e The mRNA level of Grp78, Chop, ERdj4, and relative mRNA level of β-actin were determined by qRT-PCR in mice. Data are presented as means ± the standard deviation (SD) (n ≥ 3) (*P<0.05, **P<0.01, ***P<0.001)