Fig. 6.

Kinetics study of pyocyanin production through competitive inhibition by 14-deoxy-11,12-didehydroandrographolide with C4-HSL or C12-HSL. Secretion of virulent factor such as pyocyanin was estimated after 48 h of treatment with AG or DDAG individually and combined with sub-MIC dose of AZI (6 μg/mL) or GEN (4 μg/mL). a Percentage of inhibition of pyocyanin production was represented compared with untreated control. Inset represents the pyocyanin production on microbial culture petri plates after 48 h of treatment. b Pyocyanin produced by P. aeruginosa was measured after 4 h interval up to 24 h considering total eight groups, based on different drug treatments. In figure, black line represents control group without any drug treatment; red line represents bacteria treated with only AZI (6 μg/mL); light blue represents treated with only GEN (4 μg/mL); purple line represents only DDAG-treated group; green and dark blue lines represent DDAG with AZI or GEN, respectively. Each value represents as mean ± SD of three independent experiments