Table 2.
Functional comparative annotation of ORFs. The ORF MgrBr135 (accession number SRR7726101) selected to proceed with subcloning is highlighted
| ORF | RAST | Blastp | Pfam |
|---|---|---|---|
| 1 | Regulator protein | Regulator protein | Regulator protein |
| 2 | Cell division protein FtsH | Cell division protein FtsH | Cell division protein FtsH |
| 3 | 1-Deoxy-d-xylulose 5-phosphate reductoisomerase | 1-Deoxy-d-xylulose 5-phosphate reductoisomerase | 1-Deoxy-d-xylulose 5-phosphate reductoisomerase |
| 4 | Intramembrane protease | Probable metalloproteinase | - |
| 5 | UDP-3-O-[3-Hydroxymyristoyl] glucosamine N-acyltransferase | UDP-3-O-(3-Hydroxymyristoyl) glucosamine N-acyltransferase | UDP-3-O-(3-Hydroxymyristoyl) glucosamine N-acyltransferase |
| 6 | Hypothetical protein | Hypothetical protein | Hypothetical protein |
| 7 | Hypothetical protein | Hypothetical protein | Hypothetical protein |
| 8 | Acetoacetate metabolism regulatory | Acetoacetate metabolism regulatory | Acetoacetate metabolism regulatory |
| 9 | Ribonucleotide reductase of class II | Ribonucleotide reductase | Ribonucleotide reductase |
| 10 | Ribonucleotide reductase of class II | Ribonucleotide reductase | Ribonucleotide reductase |
| 11 | Ribosomal protein | Ribosomal protein | Ribosomal protein |
| 12 | Ribonuclease | Ribonuclease | Ribonuclease |
| 13 | Hypothetical protein | Hypothetical protein | - |
| 14 | Hypothetical protein | Hypothetical protein | - |
| 15 | Phosphoenolpyruvate protein | Phosphoenolpyruvate protein | Phosphoenolpyruvate protein |
| 16 | Phosphotransferase system, phosphocarrier protein HPr | PTS IIA–like nitrogen regulatory protein PtsN | - |
| 17 | Probable sigma-54 modulation protein | Probable sigma-54 modulation protein | Probable sigma-54 modulation protein |
| 18 | Iron-binding protein | Iron transporter | Iron transporter |
| 19 | Hypothetical protein | Hypothetical protein | - |
| 20 | Serine hydroxymethyltransferase | Glycine hydroxymethyltransferase | Serine hydroxymethyltransferase |
| 21 | Hypothetical protein | Hypothetical protein | Hypothetical protein |
| 22 | Hypothetical protein | Hypothetical protein | Hypothetical protein |
| 23 | Hypothetical protein | Hypothetical protein | - |
| 24 | Alpha/beta hydrolase fold | Alpha/beta hydrolase fold protein | Alpha/beta hydrolase fold protein |
| 25 | Hypothetical protein | Hypothetical protein | - |
| 26 | Hypothetical protein | Hypothetical protein | Hypothetical protein |
| 27 | Radical SAM domain protein | Radical SAM protein | - |
| 28 | Hypothetical protein | Hypothetical protein | Hypothetical protein |
| 29 | Trehalose synthase | Alpha-amylase | Phosphotransferase enzyme family |
| 30 | 1,4-Alpha-glucan branching enzyme | Glycogen branching protein | Alpha amylase |