Table 1.

Molecular and phenotypic characterization of L. lactis subsp. lactis isolates targeting the identification of L. lactis subsp. lactis bv. diacetylactis

Isolate	Sample origin	Identification protocol1			Identification
		PCR2	citrate	diacetyl
LVA2.1	Cow milk	+	+	+	L. lactis subsp. lactis bv. diacetylactis
LVA2.2	Cow milk	+	+	+	L. lactis subsp. lactis bv. diacetylactis
LVA2VACA	Cow milk	+	+	+	L. lactis subsp. lactis bv. diacetylactis
LCA1	Goat milk	+	+	+	L. lactis subsp. lactis bv. diacetylactis
LCA2	Goat milk	+	+	+	L. lactis subsp. lactis bv. diacetylactis
LCA4	Goat milk	−	−	−	−
LCA5	Goat milk	+	+	+	L. lactis subsp. lactis bv. diacetylactis
BUF1	Buffalo milk	+	−	−	−
LVTC8MRS	Cream milk (cow)	−	+	+	−
Q1C2	Artisanal cheese (Amazon)	−	−	+	−
Q1C4	Artisanal cheese (Amazon)	−	+	+	−
Q1C5	Artisanal cheese (Amazon)	+	+	+	L. lactis subsp. lactis bv. diacetylactis
Q1C7	Artisanal cheese (Amazon)	+	+	+	L. lactis subsp. lactis bv. diacetylactis
Q1C10	Artisanal cheese (Amazon)	+	+	+	L. lactis subsp. lactis bv. diacetylactis
Q4C8	Artisanal cheese (Amazon)	+	+	+	L. lactis subsp. lactis bv. diacetylactis
Q5C6	Artisanal cheese (Amazon)	+	+	+	L. lactis subsp. lactis bv. diacetylactis
Q6C2	Artisanal cheese (Amazon)	−	−	−	−
Q13C4	Artisanal cheese (Marajó)	−	−	+	−
Q15C3	Artisanal cheese (Marajó)	+	+	+	L. lactis subsp. lactis bv. diacetylactis
SAM12	Peanut silage (dairy farm)	+	+	+	L. lactis subsp. lactis bv. diacetylactis
SBR1	Grass silage (dairy farm)	+	+	+	L. lactis subsp. lactis bv. diacetylactis
SBR3	Grass silage (dairy farm)	+	−	+	-
SBR4	Grass silage (dairy farm)	+	+	+	L. lactis subsp. lactis bv. diacetylactis

¹Identification protocol described in the Material and methods section; ²PCR product of 934 bp was indicative as typical of L. lactis subsp. lactis bv. diacetylactis, as described by Beimfohr et al. [15]; Results: +, positive; −, negative