Table 1.

Parameterization of gates

	Response functiona				Promoter interferencea		Kineticsa
	y min	y max	K	n	Non‐specific (α)	Roadblocking (β)b	Induction (${\mathbf{\tau }}_{\mathbf{y}}^{\mathbf{ON}}$)	Relaxation (${\mathbf{\tau }}_{\mathbf{y}}^{\mathbf{OFF}}$)
F1‐AmeR_2	0.29	4.6	0.12	1.5	0.09	1.00	8.00	2.30
F2‐AmeR_2	0.21	3.7	0.04	1.3	0.10	1.00	8.00	2.50
N1‐LmrA_2	0.077	1.3	0.09	1.8	0.28	1.00	1.00	1.30
A1‐AmtR_2	0.035	3.1	0.05	1.7	0.27	1.00	0.90	2.50
H1‐HlyIIR_2	0.004	2.1	0.13	2.6	0.15	1.00	0.45	4.00
P1‐PhlF	0.004	6.9	0.04	3.8	0.22	0.06	0.30	4.00
P2‐PhlF	0.007	7.5	0.21	4.5	0.15	0.12	0.20	4.00
P3‐PhlF	0.004	7.1	0.12	3.3	0.24	0.06	0.15	5.00
S1‐SrpR	0.001	1.4	0.02	3.1	0.50	0.05	5.00	7.00
S2‐SrpR	0.003	3.2	0.06	2.7	0.35	0.09	0.50	7.00
S3‐SrpR	0.004	3.1	0.09	2.7	0.33	0.10	0.70	6.00
S4‐SrpR	0.004	3.2	0.11	2.7	0.38	0.09	1.80	8.00
E1‐BetI_2	0.041	2.8	0.28	2.9	0.64	0.46	0.40	1.50
B1‐BM3R1	0.004	0.6	0.06	2.9	0.64	0.05	0.90	1.10
B2‐BM3R1	0.006	0.5	0.57	3.8	0.71	0.05	0.90	1.10
B3‐BM3R1	0.005	0.6	0.21	3.1	0.74	0.05	0.50	2.00
C1‐CymR	0.010	3.0	0.10	3.7	0.10	0.07	0.50	2.50
V1‐VanR	0.043	6.2	0.05	2.9	0.12	0.32	0.40	11.0

^aThe maximum allowed value is one during fitting.

^bThe units of y _min and y _max are RPU. The units of K are RPU. The timescales are in units of 1/h. n, α, and β are unitless.