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. 2020 Feb 26;10(2):190306. doi: 10.1098/rsob.190306

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© 2020 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 7. — Impact of CAPAM knockdown on cap dinucleotide abundance. HeLa cells were transfected with scrambled siRNA or CAPAM siRNA for 3 days. (a) Western blot of CAPAM and actin (from re-probing) in siRNA-treated HeLa cells. (b) Abundance of mRNA cap dinucleotides in siRNA-treated HeLa cells. Samples were compared by an ANOVA, with Sidak's post-test-adjusted p-values are shown. (c) The ratio of ^m7Gppp^m6A_m to ^m7GpppA_m in mRNA from siRNA-treated HeLa cells. Samples were compared by a Student's t-test.