Quantification of cytokine and chemokine levels from spleen extracts. (A) IL1α, 28 ± 1.4 sham, 39.8 ± 3.7 traumatic brain injury (TBI). (B) IL1β, 3.96 ± 0.53 sham, 2.25 ± 0.31 TBI; IL4, 8.9 ± 0.2 sham, 8.6 ± 0.14 TBI; IL6, 2.5 ± 0.22 sham, 3.7 ± 0.6 TBI; IL10, 5.8 ± 0.7 sham, 4.6 ± 0.8 TBI. (C) IP10, 79.2 ± 8.9 sham, 74.9 ± 3.9, were measured using Luminex bead based Multiplex immunoassay (n = 7–9 animals for sham or blast TBI). (D) Spleen/body weight ratios were 0.32 ± 0.009 sham, 0.27 ± 0.012 blast; n = 6 sham and 6 blast. Note: Body weight losses because of experimental paradigm were not significantly different. The % change was 6.8 ± 1.2 sham, 5.9 ± 1.1 blast. p = 0.58, n = 6 sham and 6 blast. The p values are indicated on the bar plots.